Title

Evaluation of three analyte-specific reagents for detection and typing of herpes simplex virus in cerebrospinal fluid

Document Type

Article

Publication Date

3-2009

Identifier

DOI: 10.1016/j.diagmicrobio.2008.11.013

Abstract

The performance of three analyte-specific reagents (ASRs); Cepheid herpes simplex virus (HSV) Typing Primer Probe set (CD), Eragen MultiCode-Rtx HSV-1/2 kit primer mix (ER), and Roche LightCycler HSV-1/2 Primer/Hybridization Probes (RD), was evaluated for detection and typing of herpes simplex virus (HSV-1 and HSV-2) in cerebrospinal fluid (CSF) specimens. Of 68 CSF specimens, HSV-1 was detected in 8 specimens and HSV-2 was detected in 17 specimens. ER detected all 25 HSV-positive specimens, whereas CD and RD detected 24 and 23 HSV-positive specimens, respectively. The results of HSV typing with the 3 ASRs were in complete agreement. The analytical sensitivity of all ASRs was determined to be about 101 copies/reaction. Our results demonstrate that the performances of all 3 ASRs are comparable and reliable for routine clinical testing in detection and typing of HSV DNA in CSF. © 2009 Elsevier Inc. All rights reserved.

Journal Title

Diagnostic Microbiology and Infectious Disease

Volume

63

Issue

3

First Page

286

Last Page

291

Keywords

Analyte-specific reagents, Cepheid, Eragen, Herpes simplex virus, Real-time PCR, Roche

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