Document Type

Article

Publication Date

9-8-2020

Identifier

DOI: 10.1016/j.stemcr.2020.07.018

Abstract

Mouse embryonic stem cells (ESCs) cultured in defined medium resemble the pre-implantation epiblast in the ground state, with full developmental capacity including the germline. β-Catenin is required to maintain ground state pluripotency in mouse ESCs, but its exact role is controversial. Here, we reveal a Tcf3-independent role of β-catenin in restraining germline and somatic lineage differentiation genes. We show that β-catenin binds target genes with E2F6 and forms a complex with E2F6 and HMGA2 or E2F6 and HP1γ. Our data indicate that these complexes help β-catenin restrain and fine-tune germ cell and neural developmental potential. Overall, our data reveal a previously unappreciated role of β-catenin in preserving lineage differentiation integrity in ground state ESCs.

Journal Title

Stem Cell Reports

Volume

15

Issue

3

First Page

662

Last Page

676

Keywords

ESC ground state maintenance; Wnt signaling; germline; ground state; lineage differentiation; mouse embryogenesis; naive pluripotency

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