Title

25-Hydroxyvitamin D Testing: Immunoassays Versus Tandem Mass Spectrometry.

Document Type

Article

Publication Date

9-2018

Identifier

DOI: 10.1016/j.cll.2018.05.007

Abstract

Vitamin D has been associated with many health conditions. Because of widespread deficiency in the general population, laboratory testing of vitamin D has increased exponentially in recent years. Currently, 25-hydroxyvitamin D (25[OH]D) is considered the best marker of vitamin D status. Automated immunoassays and tandem mass spectrometry are the most widely used assays for the measurement of 25(OH)D. Because a medical decision of vitamin D deficiency and treatment are made based on specific levels, it is important that different 25(OH)D assays are harmonized. Despite standardization efforts, significant differences remain among various methods and laboratories for the measurement of 25(OH)D.

Journal Title

Clinics in laboratory medicine

Volume

38

Issue

3

First Page

439

Last Page

453

MeSH Keywords

Chromatography, High Pressure Liquid; Clinical Chemistry Tests; Humans; Immunoassay; Tandem Mass Spectrometry; Vitamin D

Keywords

25-Hydroxyvitamin D; 3-Epi-25-hydroxyvitamin D; HPLC-MS/MS; HPLC-tandem mass spectrometry; Immunoassay; Protein-binding assay; Vitamin D; Vitamin D binding-protein

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