Title

Cepheid Xpert Xpress Flu/RSV evaluation performed by minimally trained non-laboratory operators in a CLIA-waived environment.

Document Type

Article

Publication Date

10-2022

Identifier

DOI: 10.1016/j.diagmicrobio.2022.115764; PMCID: PMC9271353

Abstract

The COVID-19 pandemic highlighted the significance of readily available and easily performed viral testing for surveillance during future infectious pandemics. The objectives of this study were: to assess the performance of the Xpert Xpress Flu and/or RSV test, a multiplex PCR assay for detecting influenza A and B virus and respiratory syncytial virus nucleic acids in respiratory tract specimens, relative to the Quidel Lyra Influenza A+B assay and the Prodesse ProFlu+ assay, and the system's ease of use by minimally trained operators. Overall, the Xpert Xpress Flu/RSV test demonstrated a high positive and negative percent agreement with the comparator assays, and was easy to use and interpret results, based on the operators' feedback. We concluded that the Xpert Xpress Flu/RSV test is sensitive, specific, and easy to use for the diagnosis of influenza and RSV by minimally trained operators and can be a valuable tool in future infectious clusters or pandemics.

Journal Title

Diagnostic microbiology and infectious disease

Volume

104

Issue

2

First Page

115764

Last Page

115764

MeSH Keywords

COVID-19; Humans; Influenza A virus; Influenza B virus; Influenza, Human; Molecular Diagnostic Techniques; Nasopharynx; Pandemics; Real-Time Polymerase Chain Reaction; Respiratory Syncytial Virus Infections; Respiratory Syncytial Virus, Human; Sensitivity and Specificity

Keywords

CLIA-Waved Environment; Influenza; Multiplex real-time PCR test; RSV

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