Title

Measurement of urea nitrogen and creatinine concentrations in peritoneal dialysate and other body fluids using the Vitros analyzer.

Document Type

Article

Publication Date

3-1-2007

Identifier

DOI: 10.1016/j.clinbiochem.2006.12.009

Abstract

OBJECTIVES: A majority of chemistry analyzers have separate modes for estimating urea nitrogen and creatinine in serum and urine because of significant differences in their concentration. As there is no defined mode for other biological fluids such as dialysis fluid, these can be run on either of the two modes. On Vitros (Clinical Ortho-Diagnostics, Rochester, NY) chemistry analyzer, we observed an overestimation in calculated creatinine clearance (CrCl) and Kt/V(urea) in some children that led us to investigate into methodology used for these assays.

DESIGN AND METHODS: We measured urea nitrogen and creatinine on peritoneal dialysate effluent, pleural fluid and hemofiltration effluent on serum and urine modes of Vitros analyzer.

RESULTS: Vitros chemistry analyzer significantly overestimated urea nitrogen and creatinine in 'urine' mode on samples with low urea nitrogen and creatinine.

CONCLUSIONS: Since peritoneal dialysate effluent has concentrations of urea nitrogen and creatinine comparable to serum; hence, it should be analyzed on 'serum' mode.

Journal Title

Clinical biochemistry

Volume

40

Issue

5-6

First Page

420

Last Page

422

MeSH Keywords

Body Fluids; Chemistry, Clinical; Creatinine; Dialysis Solutions; Humans; Nitrogen; Peritoneal Dialysis; Reproducibility of Results

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