Title

Multicenter clinical evaluation of the novel Alere™ i Influenza A&B isothermal nucleic acid amplification test

Document Type

Article

Publication Date

9-2014

Identifier

DOI: 10.1016/j.jcv.2014.06.001

Abstract

Background: Rapid detection of influenza infection is important for patient management and timely anti-viral therapy. Rapid antigen detection tests for influenza have inferior sensitivity when compared to nucleic acid-based amplification tests. An isothermal nucleic acid amplification test that offers the potential for rapid molecular testing at the clinical point-of-care with simple equipment can improve influenza detection rates.

Objectives: To evaluate the performance of Alere™ i Influenza A&B isothermal nucleic acid amplification test to detect influenza A and B in comparison to viral cell culture as reference method. Study design: A prospective, multicenter, clinical study to evaluate the clinical performance of the Alere™ i Influenza A&B assay in a point-of-care setting using prospectively enrolled specimens from both children and adults was conducted in seven sites.

Results: In comparison with viral cell culture, the overall sensitivity and specificity of the Alere™ i Influenza A&B assay was 97.8% and 85.6% for the detection of influenza A, and 91.8% and 96.3% for the detection of influenza B, respectively. Following resolution of discrepant results by real-time RT-PCR the sensitivity and specificity of the Alere™ i Influenza A&B assay improved to 99.3% and 98.1% for influenza A, and 97.6% and 100% for influenza B, respectively.

Conclusions: The Alere™ i Influenza A&B isothermal nucleic acid amplification test is an ideal point-of-care test for influenza detection in children and adults due to its high sensitivity and specificity and ability to generate results within 15. min from specimen receipt. © 2014 The Authors.

Journal Title

Journal of Clinical Virology

Volume

61

Issue

1

First Page

81

Last Page

86

Keywords

Alere™ i Influenza, Influenza, Nucleic acid amplification, POC, RADT

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