Document Type
Article
Publication Date
4-18-2025
Identifier
DOI: 10.1016/j.isci.2025.112243; PMCID: PMC12005339
Abstract
Microbial succession during postnatal gut development in mice is likely impacted by site of sampling, time, intestinal injury, and host genetics. We investigated this in wild-type and Sigirr transgenic mice that encode the p.Y168X mutation identified in a neonate with necrotizing enterocolitis (NEC). Temporal profiling of the ileal and colonic microbiome after birth to weaning revealed a clear pattern of progression from a less diverse, Proteobacteria/Escherichia_Shigella dominant community to a more diverse, Firmicutes/Bacteroidetes dominant community. Formula milk feeding, a risk factor for necrotizing enterocolitis, decreased Firmicutes and increased Proteobacteria leading to enrichment of bacterial genes denoting exaggerated glycolysis and increased production of acetate and lactate. Sigirr transgenic mice exhibited modest baseline differences in microbiota composition but exaggerated formula feeding-induced dysbiosis, mucosal inflammation, and villus injury. Postnatal intestinal microbiota succession in mice resembles human neonates and is shaped by developmental maturity, ileal vs. colonic sampling, formula feeding, and Sigirr genotype.
Journal Title
iScience
Volume
28
Issue
4
First Page
112243
Last Page
112243
PubMed ID
40248118
Keywords
Cell biology; Immunology; Microbiology
Recommended Citation
Umar S, Yu W, Xuan H, et al. Neonatal gut microbiota succession in mice mapped over time, site, injury and single immunoglobulin interleukin-1 related receptor genotype. iScience. 2025;28(4):112243. Published 2025 Mar 18. doi:10.1016/j.isci.2025.112243
Comments
This article is available under the Creative Commons CC-BY-NC license and permits non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.
Publisher's Link: https://doi.org/10.1016/j.isci.2025.112243