Title

Comparative analysis of Four sample-to-answer influenza A/B and RSV nucleic acid amplification assays using adult respiratory specimens.

Document Type

Article

Publication Date

9-2019

Identifier

DOI: 10.1016/j.jcv.2019.07.003

Abstract

BACKGROUND: The use of Sample-to-answer (STA) platforms for the detection of influenza A/B and respiratory syncytial virus (RSV) have greatly improved patient care. These diagnostic assays based on nucleic acid amplification are rapid, accurate and relatively easy to perform.

OBJECTIVES: We compared four such platforms for detecting FluA, FluB, and RSV from adult respiratory specimens: Hologic Panther Fusion® Flu A/B/RSV (Fusion), Cobas® Influenza A/B & RSV (Liat), Luminex Aries® Flu A/B & RSV (Aries), and Diasorin SimplexaTM Flu A/B & RSV (Simplexa).

STUDY DESIGN: Nasopharyngeal (NP) swabs (n = 224) from adults were tested on these platforms and results were compared to Center for Disease Control and Prevention recommended real-time RT-PCR assay for influenza A/B and RSV. Subtyping for FluA and FluB was performed for discrepant analysis where applicable.

RESULTS: Of the 82 FluA, 26 FluB, 15 RSV-positive specimens tested, the positive and negative percentage agreements (PPA and NPA respectively) for FluA detection were 100/100 (Fusion), 95.1/100 (Liat), 92.5/100 (Aries), and 84.1/99.3 (Simplexa); PPA and NPA for FluB detection were 92.3/99.5 (Fusion), 96/99.5 (Liat), 100/99.5 (Aries), and 80.8/100 (Simplexa); and for RSV detection were 100/100 (Fusion), 100/100 (Liat), 88.6/99.5 (Aries), and 73.3/100 (Simplexa). 82 confirmed FluA included 23 pH1N1 and 57 H3N2 strains with 2 strains remaining untyped. Of the 26 confirmed FluB, 25 were of the Yamagata lineage and 1 of unknown lineage.

CONCLUSION: Only 2 STA platforms demonstrated >95% PPA for the detection of all three targets while all the 4 platforms demonstrated >95% NPA for FluA, FluB and RSV.

Journal Title

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

Volume

118

First Page

9

Last Page

13

MeSH Keywords

Adolescent; Adult; Aged; Aged, 80 and over; Female; Humans; Influenza A virus; Influenza B virus; Influenza, Human; Male; Middle Aged; Nasopharynx; Nucleic Acid Amplification Techniques; Respiratory Syncytial Virus Infections; Respiratory Syncytial Virus, Human; Retrospective Studies; Young Adult

Keywords

Adult; Diagnostic assays; Flu and RSV; Sample to answer

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