Evaluation of three analyte-specific reagents for detection and typing of herpes simplex virus in cerebrospinal fluid

Creator(s)

Suresh B. Selvaraju, Children's Mercy Hospitals and Clinics
Michelle Wurst, University of Kansas Medical Center
Rebecca T. Horvat, University of Kansas Medical Center
Rangaraj Selvarangan, Children's Mercy HospitalFollow

Document Type

Article

Publication Date

3-2009

Identifier

DOI: 10.1016/j.diagmicrobio.2008.11.013

Abstract

The performance of three analyte-specific reagents (ASRs); Cepheid herpes simplex virus (HSV) Typing Primer Probe set (CD), Eragen MultiCode-Rtx HSV-1/2 kit primer mix (ER), and Roche LightCycler HSV-1/2 Primer/Hybridization Probes (RD), was evaluated for detection and typing of herpes simplex virus (HSV-1 and HSV-2) in cerebrospinal fluid (CSF) specimens. Of 68 CSF specimens, HSV-1 was detected in 8 specimens and HSV-2 was detected in 17 specimens. ER detected all 25 HSV-positive specimens, whereas CD and RD detected 24 and 23 HSV-positive specimens, respectively. The results of HSV typing with the 3 ASRs were in complete agreement. The analytical sensitivity of all ASRs was determined to be about 101 copies/reaction. Our results demonstrate that the performances of all 3 ASRs are comparable and reliable for routine clinical testing in detection and typing of HSV DNA in CSF. © 2009 Elsevier Inc. All rights reserved.

Journal Title

Diagnostic Microbiology and Infectious Disease

Volume

63

Issue

3

First Page

286

Last Page

291

Keywords

Analyte-specific reagents, Cepheid, Eragen, Herpes simplex virus, Real-time PCR, Roche

Recommended Citation

Selvaraju, S. B., Wurst, M., Horvat, R. T., Selvarangan, R. Evaluation of three analyte-specific reagents for detection and typing of herpes simplex virus in cerebrospinal fluid Diagnostic Microbiology and Infectious Disease 63, 286-291 (2009).