Characterization of Reference Materials for Genetic Testing of CYP2D6 Alleles: A GeT-RM Collaborative Project.
Document Type
Article
Publication Date
11-2019
Identifier
DOI: 10.1016/j.jmoldx.2019.06.007; PMCID: PMC6854474 (available on 2020-11-01)
Abstract
Pharmacogenetic testing increasingly is available from clinical and research laboratories. However, only a limited number of quality control and other reference materials currently are available for the complex rearrangements and rare variants that occur in the CYP2D6 gene. To address this need, the Division of Laboratory Systems, CDC-based Genetic Testing Reference Material Coordination Program, in collaboration with members of the pharmacogenetic testing and research communities and the Coriell Cell Repositories (Camden, NJ), has characterized 179 DNA samples derived from Coriell cell lines. Testing included the recharacterization of 137 genomic DNAs that were genotyped in previous Genetic Testing Reference Material Coordination Program studies and 42 additional samples that had not been characterized previously. DNA samples were distributed to volunteer testing laboratories for genotyping using a variety of commercially available and laboratory-developed tests. These publicly available samples will support the quality-assurance and quality-control programs of clinical laboratories performing CYP2D6 testing.
Journal Title
The Journal of molecular diagnostics : JMD
Volume
21
Issue
6
First Page
1034
Last Page
1052
MeSH Keywords
Alleles; Cytochrome P-450 CYP2D6; Genetic Variation; Genotyping Techniques; Haplotypes; High-Throughput Nucleotide Sequencing; Humans; Intersectoral Collaboration; Multiplex Polymerase Chain Reaction; Real-Time Polymerase Chain Reaction; Reference Standards
Keywords
Alleles; Cytochrome P-450 CYP2D6; Genetic Variation; Genotyping Techniques; Haplotypes; High-Throughput Nucleotide Sequencing; Humans; Intersectoral Collaboration; Multiplex Polymerase Chain Reaction; Real-Time Polymerase Chain Reaction; Reference Standards
Recommended Citation
Gaedigk A, Turner A, Everts RE, et al. Characterization of Reference Materials for Genetic Testing of CYP2D6 Alleles: A GeT-RM Collaborative Project. J Mol Diagn. 2019;21(6):1034-1052. doi:10.1016/j.jmoldx.2019.06.007
COinS
Comments
Grant support