Development of a blocking enzyme-linked immunosorbent assay for detection of serum antibodies to O157 antigen of Escherichia coli.

Creator(s)

W Laegreid
M HoffmanFollow
J Keen
R Elder
J Kwang

Document Type

Article

Publication Date

3-1-1998

Identifier

PMID: 9521150, PMCID: PMC121365

Abstract

The O157 antigen of Escherichia coli shares structural elements with lipopolysaccharide (LPS) antigens of other bacterial species, notably Brucella abortus and Yersinia enterocolitica 09, a fact that confounds the interpretation of assays for anti-O157 antibodies. To address this problem, a blocking enzyme-linked immunosorbent assay (bELISA) was designed with E. coli O157:H7 LPS as the antigen and a monoclonal antibody specific for E. coli O157, designated 13B3, as the competing antibody. The bELISA had equivalent sensitivity to, and significantly higher specificity than, the indirect ELISA (iELISA), detecting anti-O157 antibodies in sera from cattle experimentally inoculated with O157:H7. Only 13% of sera from naive heifers vaccinated for or experimentally infected with B. abortus had increased anti-O157 bELISA titers, while 61% of anti-O157 iELISA titers were increased. The bELISA is a sensitive and specific method for the detection of serum antibodies resulting from exposure to E. coli O157.

Journal Title

Clinical and diagnostic laboratory immunology

Volume

5

Issue

2

First Page

242

Last Page

246

MeSH Keywords

Animals; Antibodies; Bacterial; Antigens; Bacterial; Cattle; Enzyme-Linked Immunosorbent Assay; Escherichia coli Infections; Escherichia coli O157; Sensitivity and Specificity

Keywords

bELISA; E. Coli O157; Cows

Comments

http://cvi.asm.org/content/5/2/242.long

Recommended Citation

Laegreid, W., Hoffman, M., Keen, J., Elder, R., Kwang, J. Development of a blocking enzyme-linked immunosorbent assay for detection of serum antibodies to O157 antigen of Escherichia coli. Clinical and diagnostic laboratory immunology 5, 242-246 (1998).