Document Type
Article
Publication Date
11-9-2020
Identifier
DOI: 10.1038/s41597-020-00719-4; PMCID: PMC7652884
Abstract
Both poly(A) enrichment and ribosomal RNA depletion are commonly used for RNA sequencing. Either has its advantages and disadvantages that may lead to biases in the downstream analyses. To better access these effects, we carried out both ribosomal RNA-depleted and poly(A)-selected RNA-seq for CD4+ T naive cells isolated from 40 healthy individuals from the Blueprint Project. For these 40 individuals, the genomic and epigenetic data were also available. This dataset offers a unique opportunity to understand how library construction influences differential gene expression, alternative splicing and molecular QTL (quantitative loci) analyses for human primary cells.
Journal Title
Sci Data
Volume
7
Issue
1
First Page
376
Last Page
376
Recommended Citation
Chen L, Yang R, Kwan T, et al. Paired rRNA-depleted and polyA-selected RNA sequencing data and supporting multi-omics data from human T cells. Sci Data. 2020;7(1):376. Published 2020 Nov 9. doi:10.1038/s41597-020-00719-4
Comments
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Publisher's Link: https://doi.org/10.1038/s41597-020-00719-4