Role of cochlear synaptopathy in cytomegalovirus infected mice and in children.
Document Type
Article
Publication Date
11-2020
Identifier
DOI: 10.1016/j.ijporl.2020.110275
Abstract
OBJECTIVES: Determine whether a murine model of cytomegalovirus (CMV) and CMV- infected children show evidence of synaptopathy.
STUDY DESIGN: Murine model of CMV infection and case series.
SUBJECTS AND METHODS: C57 BL/6 mice were inoculated with murine-CMV (mCMV). Auditory function was assessed using Auditory Brainstem Response (ABR) and distortion product otoacoustic emission (DPOAE) testing. Temporal bones from mCMV-infected mice were used for both ribbon synapse and hair cell quantification. Four groups of children (non-CMV normal hearing, non-CMV hearing impaired, CMV normal hearing and CMV hearing impaired) underwent ABRs between 2014 and 2018. The outcomes included raw amplitude, wave I:V amplitude ratio, absolute latency, and interpeak latency.
RESULTS: Mice at 8 weeks post mCMV infection had higher ABR and DPOAE (P < 0.05) thresholds and increased outer hair cell loss compared to uninfected mice and mCMV-infected mice at 4 and 6 weeks post infection, indicating progressive hearing loss. A reduction in the wave I amplitude and synaptic counts were noted earlier at 4 weeks in CMV-infected mice (P < 0.05). The human data indicated that the wave I:V amplitude ratio was lower on average in CMV-infected groups when compared to the uninfected cohorts. The wave I:V amplitude ratio for the click and 4k stimuli were not significantly different between the congenital CMV-infected and uninfected children with normal or with hearing loss.
CONCLUSION: This study suggests mCMV infection results in a synaptopathy before hair cell damage. Additional studies need to be performed to determine whether this effect is also observed in CMV-infected children.
LEVEL OF EVIDENCE: Animal studies and basic science- NA; human studies: level 4.
Journal Title
International journal of pediatric otorhinolaryngology
Volume
138
First Page
110275
Last Page
110275
MeSH Keywords
Animals; Auditory Threshold; Cochlea; Cytomegalovirus; Cytomegalovirus Infections; Evoked Potentials, Auditory, Brain Stem; Mice; Otoacoustic Emissions, Spontaneous
Keywords
Cytomegalovirus; Murine; Sensorineural hearing loss; Synaptopathy
Recommended Citation
Almishaal A, Mathur PD, Franklin L, et al. Role of cochlear synaptopathy in cytomegalovirus infected mice and in children. Int J Pediatr Otorhinolaryngol. 2020;138:110275. doi:10.1016/j.ijporl.2020.110275
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