Development of a UPLC-MS/MS method for quantitation of metronidazole and 2-hydroxy metronidazole in human plasma and its application to a pharmacokinetic study.

Document Type

Article

Publication Date

8-15-2018

Identifier

DOI: 10.1016/j.jchromb.2018.06.024

Abstract

An ultra-performance liquid-chromatography mass-spectrometry (UPLC-MS/MS) method for simultaneous quantitation of metronidazole and 2-hydroxymetronidazole in human plasma was developed and validated. Metronidazole and 2-hydroxymetronidazole were extracted from a small volume of human plasma (10 μL) by hydrophilic lipophilic balanced solid phase extraction on 96-well μ-elution plates. Chromatographic separation of analytes was achieved on an Acquity UPLC BEH C18 column (1.7 μm, 2.1 × 100 mm) using gradient elution with a blend of 0.1% formic acid in water and 0.1% formic acid in methanol at a flow rate of 0.25 mL/min. Mass spectrometric detection was achieved using multiple reaction monitoring (MRM) in positive-ion electrospray-ionization (ESI) mode. Ion transitions were optimized at m/z 171.85->127.9 for metronidazole and m/z 187.9->125.9 for 2-hydroxymetronidazole. The assay was linear for both analytes over the concentration range of 0.1-300 μM; intra- and inter-assay precisions and accuracies were

Journal Title

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Volume

1092

First Page

272

Last Page

278

MeSH Keywords

Adolescent; Adult; Chromatography, High Pressure Liquid; Drug Stability; Humans; Limit of Detection; Linear Models; Metronidazole; Middle Aged; Reproducibility of Results; Tandem Mass Spectrometry; Young Adult

Keywords

2-Hydoxymetronidazole; Human plasma; Mass spectrometry; Metronidazole; Pharmacokinetics; UPLC-MS/MS

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