A Simple Liquid Chromatography Tandem Mass Spectrometry Method for Quantitation of Plasma Busulfan.

Creator(s)

Shuang Deng, Children's Mercy Hospital
Michael Kiscoan, Children's Mercy HospitalFollow
C. Clinton Frazee III, Children's Mercy HospitalFollow
Susan M. Abdel-Rahman, Children's Mercy HospitalFollow
Jignesh Dalal, Children's Mercy Hospital
Uttam Garg, Children's Mercy HospitalFollow

Document Type

Article

Publication Date

1-1-2016

Identifier

DOI: 10.1007/978-1-4939-3252-8_9

Abstract

Busulfan is an alkylating agent widely used in the ablation of bone marrow cells before hematopoietic stem cell transplant. Due to large intraindividual and interindividual variations, and narrow therapeutic window, therapeutic drug monitoring of busulfan is warranted. A quick and reliable HPLC-MS/MS method was developed for the assay of plasma busulfan. HPLC involved C18 column, and MS/MS was used in electrospray ionization (ESI) positive mode. Quantitation and identification of busulfan was made using various multiple reactions monitoring (MRMs). Isotopic labeled busulfan-d8 was used as the internal standard. The method is linear from 50 to 2500 ng/mL and has with-in run and between-run imprecision of%.

Journal Title

Methods in molecular biology (Clifton, N.J.)

Volume

1383

First Page

79

Last Page

87

MeSH Keywords

Busulfan; Chromatography, High Pressure Liquid; Drug Monitoring; Humans; Immunosuppressive Agents; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry

Keywords

Bone marrow transplant; Busulfan; Leukemia; Liquid chromatography; Mass spectrometry; BMT

Recommended Citation

Deng S, Kiscoan M, Frazee C, Abdel-Rahman S, Dalal J, Garg U. A Simple Liquid Chromatography Tandem Mass Spectrometry Method for Quantitation of Plasma Busulfan. Methods Mol Biol. 2016;1383:79-87. doi:10.1007/978-1-4939-3252-8_9