Cetirizine Quantification by High-Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).
Document Type
Article
Publication Date
1-1-2016
Identifier
DOI: 10.1007/978-1-4939-3252-8_13
Abstract
A multiple reaction monitoring (MRM), positive ion electrospray ionization, LC/MS/MS method is described for the quantification of cetirizine. The compound was isolated from human plasma by protein precipitation using acetonitrile. Cetirizine d4 was used as an internal standard. Chromatographic conditions were achieved using a C18 column and a combination of ammonium acetate, water, and methanol as the mobile phase. MRMs were: cetirizine, 389.26 → 165.16, 201.09; cetirizine d4, 393.09 → 165.15, 201.10. Calibration curves were constructed by plotting the peak area ratios of the calibrators' target MRM transition area to labeled internal standard target MRM transition area versus concentration.
Journal Title
Methods in molecular biology (Clifton, N.J.)
Volume
1383
First Page
115
Last Page
120
MeSH Keywords
Anti-Allergic Agents; Cetirizine; Chromatography, High Pressure Liquid; Drug Monitoring; Histamine H1 Antagonists, Non-Sedating; Humans; Tandem Mass Spectrometry
Keywords
Allergic rhinitis and chronic urticaria; Antihistamine; Cetirizine; H1-receptor
Recommended Citation
Munar A, Frazee C, Jones B, Garg U. Cetirizine Quantification by High-Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Methods Mol Biol. 2016;1383:115-120. doi:10.1007/978-1-4939-3252-8_13
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