Title

Cetirizine Quantification by High-Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).

Document Type

Article

Publication Date

1-1-2016

Identifier

DOI: 10.1007/978-1-4939-3252-8_13

Abstract

A multiple reaction monitoring (MRM), positive ion electrospray ionization, LC/MS/MS method is described for the quantification of cetirizine. The compound was isolated from human plasma by protein precipitation using acetonitrile. Cetirizine d4 was used as an internal standard. Chromatographic conditions were achieved using a C18 column and a combination of ammonium acetate, water, and methanol as the mobile phase. MRMs were: cetirizine, 389.26 → 165.16, 201.09; cetirizine d4, 393.09 → 165.15, 201.10. Calibration curves were constructed by plotting the peak area ratios of the calibrators' target MRM transition area to labeled internal standard target MRM transition area versus concentration.

Journal Title

Methods in molecular biology (Clifton, N.J.)

Volume

1383

First Page

115

Last Page

120

MeSH Keywords

Anti-Allergic Agents; Cetirizine; Chromatography, High Pressure Liquid; Drug Monitoring; Histamine H1 Antagonists, Non-Sedating; Humans; Tandem Mass Spectrometry

Keywords

Allergic rhinitis and chronic urticaria; Antihistamine; Cetirizine; H1-receptor

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