Document Type

Article

Publication Date

8-1-2017

Identifier

DOI: 10.1002/jor.23467

Abstract

To date, many osteochondral regenerative approaches have utilized varied combinations of biocompatible materials and cells to engineer cartilage. Even in cell-based approaches, to date, no study has utilized stem cell aggregates alone for regenerating articular cartilage. Thus, the purpose of this study was to evaluate the performance of a novel stem cell-based aggregate approach in a fibrin carrier to regenerate osteochondral defects in the Sprague-Dawley rat trochlear groove model. Two different densities of rat bone marrow mesenchymal stem cell (rBMSC) aggregates were fabricated by the hanging drop technique. At 8 weeks, the cell aggregates supported the defects and served as a catalyst for neo-cartilage synthesis, and the experimental groups may have been beneficial for bone and cartilage regeneration compared to the fibrin-only control and sham groups, as evidenced by histological assessment. The cell density of rBMSC aggregates may thus directly impact chondrogenesis. The usage of cell aggregates with fibrin as a cell-based technology is a promising and translational new treatment strategy for repair of cartilage defects. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1606-1616, 2017.

Journal Title

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Volume

35

Issue

8

First Page

1606

Last Page

1616

MeSH Keywords

Animals; Cartilage, Articular; Cell Aggregation; Fibrin; Male; Mesenchymal Stem Cell Transplantation; Pilot Projects; Primary Cell Culture; Rats, Sprague-Dawley; Regeneration

Keywords

aggregates; fibrin; osteochondral; rBMSC; trochlear groove

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