Simultaneous quantification of trimethoprim metabolites in pediatric plasma.

Creator(s)

Robert T. Tessman, Children's Mercy HospitalFollow
Whitney M. Nolte, Children's Mercy HospitalFollow
Paul C. Toren, Children's Mercy HospitalFollow
Kim T. Gibson, Children's Mercy HospitalFollow
Linda Vu, Children's Mercy HospitalFollow
Jennifer Goldman, Children's Mercy Kansas CityFollow

Document Type

Article

Publication Date

5-15-2022

Identifier

DOI: 10.1016/j.jchromb.2022.123232

Abstract

The dual agent antibiotic, trimethoprim/sulfamethoxazole (TMP-SMX), has been prescribed to treat or prevent infections for over 50 years. However, there are no published validated analytical methods for the measurement of TMP metabolites in humans. We developed methodology enabling reliable quantification of TMP and 5 metabolites in human plasma. Chromatographic separation was achieved in less than 8 min using a biphenyl column. Analytes were detected in positive electrospray mode using a tandem Waters Xevo-TQ-XS mass spectrometer. Precision and accuracy values for all analytes were within 15% of nominal values during assay validation.

Journal Title

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Volume

1198

First Page

123232

Last Page

123232

Keywords

Idiosyncratic adverse drug reactions; Metabolism; Tandem mass spectrometry; Trimethoprim; Validated methodology

Recommended Citation

Tessman RT, Nolte W, Toren P, Gibson K, Vu L, Goldman J. Simultaneous quantification of trimethoprim metabolites in pediatric plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2022;1198:123232. doi:10.1016/j.jchromb.2022.123232