Cepheid Xpert Xpress Flu/RSV evaluation performed by minimally trained non-laboratory operators in a CLIA-waived environment.
Document Type
Article
Publication Date
10-2022
Identifier
DOI: 10.1016/j.diagmicrobio.2022.115764; PMCID: PMC9271353
Abstract
The COVID-19 pandemic highlighted the significance of readily available and easily performed viral testing for surveillance during future infectious pandemics. The objectives of this study were: to assess the performance of the Xpert Xpress Flu and/or RSV test, a multiplex PCR assay for detecting influenza A and B virus and respiratory syncytial virus nucleic acids in respiratory tract specimens, relative to the Quidel Lyra Influenza A+B assay and the Prodesse ProFlu+ assay, and the system's ease of use by minimally trained operators. Overall, the Xpert Xpress Flu/RSV test demonstrated a high positive and negative percent agreement with the comparator assays, and was easy to use and interpret results, based on the operators' feedback. We concluded that the Xpert Xpress Flu/RSV test is sensitive, specific, and easy to use for the diagnosis of influenza and RSV by minimally trained operators and can be a valuable tool in future infectious clusters or pandemics.
Journal Title
Diagnostic microbiology and infectious disease
Volume
104
Issue
2
First Page
115764
Last Page
115764
MeSH Keywords
COVID-19; Humans; Influenza A virus; Influenza B virus; Influenza, Human; Molecular Diagnostic Techniques; Nasopharynx; Pandemics; Real-Time Polymerase Chain Reaction; Respiratory Syncytial Virus Infections; Respiratory Syncytial Virus, Human; Sensitivity and Specificity
Keywords
CLIA-Waved Environment; Influenza; Multiplex real-time PCR test; RSV
Recommended Citation
Shihabuddin BS, Faron ML, Relich RF, et al. Cepheid Xpert Xpress Flu/RSV evaluation performed by minimally trained non-laboratory operators in a CLIA-waived environment. Diagn Microbiol Infect Dis. 2022;104(2):115764. doi:10.1016/j.diagmicrobio.2022.115764
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