Title

Quantitation of Neuroblastoma Markers Homovanillic Acid (HVA) and Vanillylmandelic Acid (VMA) in Urine by Gas Chromatography-Mass Spectrometry (GC/MS).

Document Type

Article

Publication Date

2022

Identifier

DOI: 10.1007/978-1-0716-2565-1_17

Abstract

Neuroblastoma and other neural crest tumors can be characterized by the increased production and excretion of catecholamines and their metabolites. Homovanillic acid (HVA) and vanillylmandelic acid (VMA) are important catecholamine metabolites that can be measured to provide relatively rapid laboratory diagnosis and clinical follow-up of neuroblastoma. We present a procedure to quantify HVA and VMA in urine samples which have been diluted to a creatinine concentration of 2 mg/dL. Diluted samples are spiked with deuterated internal standards, acidified, and extracted with an organic solvent. A bis(trimethylsilyl)trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS) and pyridine mixture is added to the dried extract to create trimethylsilyl derivatives of HVA and VMA. The derivatized compounds are measured using gas chromatography-mass spectrometry (GC/MS).

Journal Title

Methods in molecular biology (Clifton, N.J.)

Volume

2546

First Page

185

Last Page

194

MeSH Keywords

Biomarkers; Catecholamines; Creatinine; Gas Chromatography-Mass Spectrometry; Homovanillic Acid; Humans; Neuroblastoma; Pyridines; Solvents; Vanilmandelic Acid

Keywords

Catecholamines; Dopamine; Epinephrine; Gas chromatography–mass spectrometry; Homovanillic acid; Neuroblastoma; Norepinephrine; Vanillylmandelic acid

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