Title

Quantification of Tryptophan, Indole, and Indoxyl Sulfate in Urine Using Liquid Chromatography-Tandem Mass Spectrometry.

Document Type

Article

Publication Date

2022

Identifier

DOI: 10.1007/978-1-0716-2565-1_44

Abstract

Having a diverse gut microbiota has been correlated with the short- and long-term success of allogeneic stem cell transplantation. Intestinal bacteria metabolize the amino acid tryptophan to indole. Indole is further oxidized and sulfonated in the liver to 3-indoxyl sulfate (3-IS), which is then excreted in urine. Urinary 3-IS is a potential biomarker for intestinal health and an early predictor of successful stem cell transplantation. We describe a rapid method for quantifying tryptophan, indole, and 3-indoxyl sulfate in urine specimens, in which urine samples are diluted with a formic acid solution and deuterated internal standards, and then injected on LC-MS/MS for analysis.

Journal Title

Methods in molecular biology (Clifton, N.J.)

Volume

2546

First Page

493

Last Page

500

MeSH Keywords

Chromatography, Liquid; Indican; Indoles; Tandem Mass Spectrometry; Tryptophan

Keywords

Allogeneic stem cell transplantation; Graft-versus-host disease; Gut microbiota; Indoxyl sulfate; LC-MS/MS; Tryptophan

Library Record

Share

COinS