Document Type

Article

Publication Date

12-16-2022

Identifier

DOI: 10.1016/j.xpro.2022.101918; PMCID: PMC9763742

Abstract

Genome-wide mapping of transcription factors (TFs) is critical to understand their functions. In chromatin immunoprecipitation (ChIP)-seq assay, it's challenging to study recruitment of low-abundant TFs transiently boud to the genome. Here, we present an optimized protocol using ChIP Next-Gen Seq Sepharose (Staph-seq) to efficiently pull down chromatin complexes. The double size selection promotes sensitive capture of genome-wide protein-DNA associations while eliminating potential Staph A contamination, which is a common problem in protocols using Staph A cells. For complete details on the use and execution of this protocol, please refer to Tao et al. (2020).

Journal Title

STAR Protoc

Volume

3

Issue

4

First Page

101918

Last Page

101918

Keywords

Chromatin immunoprecipitation (ChIP); High Throughput Screening; Molecular Biology

Comments

This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed.

Publisher's Link: https://www.sciencedirect.com/science/article/pii/S2666166722007985?via%3Dihub

Download

Share

COinS