Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection.

Document Type

Article

Publication Date

6-23-2023

Identifier

DOI: 10.3791/64274

Abstract

Tumor cells are highly motile and invasive and display altered gene expression patterns. Knowledge of how changes in gene expression regulate tumor cell migration and invasion is essential for understanding the mechanisms of tumor cell infiltration into neighboring healthy tissues and metastasis. Previously, it was demonstrated that gene knockdown followed by the impedance-based real-time measurement of tumor cell migration and invasion enables the identification of the genes required for tumor cell migration and invasion. Recently, the mRNA vaccines against SARS-CoV-2 have increased interest in using synthetic mRNA for therapeutic purposes. Here, the method using synthetic mRNA was revised to study the effect of gene overexpression on tumor cell migration and invasion. This study demonstrates that elevated gene expression with synthetic mRNA transfection followed by impedance-based real-time measurement may help identify the genes that stimulate tumor cell migration and invasion. This method paper provides important details on the procedures for examining the effect of altered gene expression on tumor cell migration and invasion.

Journal Title

J Vis Exp

Issue

196

MeSH Keywords

Humans; Cell Line, Tumor; RNA, Messenger; COVID-19 Vaccines; COVID-19; SARS-CoV-2; Transfection; Cell Movement; Neoplasm Invasiveness; Gene Expression Regulation, Neoplastic; Cell Proliferation

Keywords

Tumor Cell Line; Messenger RNA; COVID-19 Vaccines; COVID-19; SARS-CoV-2; Transfection; Cell Movement; Neoplasm Invasiveness; Neoplastic Gene Expression Regulation; Cell Proliferation

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