Cell-type profiling of the sympathetic nervous system using spatial transcriptomics and spatial mapping of mRNA.

Creator(s)

Jennifer C. Kasemeier-Kulesa
Jason A. Morrison
Sean McKinney
Hua Li
Madelaine Gogol
Kate Hall
Shiyuan Chen
Yongfu Wang
Anoja Perera
Rebecca McLennan, Children's Mercy HospitalFollow
Paul M. Kulesa

Document Type

Article

Publication Date

8-2023

Identifier

DOI: 10.1002/dvdy.577

Abstract

BACKGROUND: The molecular identification of neural progenitor cell populations that connect to establish the sympathetic nervous system (SNS) remains unclear. This is due to technical limitations in the acquisition and spatial mapping of molecular information to tissue architecture.

RESULTS: To address this, we applied Slide-seq spatial transcriptomics to intact fresh frozen chick trunk tissue transversely cryo-sectioned at the developmental stage prior to SNS formation. In parallel, we performed age- and location-matched single cell (sc) RNA-seq and 10× Genomics Visium to inform our analysis. Downstream bioinformatic analyses led to the unique molecular identification of neural progenitor cells within the peripheral sympathetic ganglia (SG) and spinal cord preganglionic neurons (PGNs). We then successfully applied the HiPlex RNAscope fluorescence in situ hybridization and multispectral confocal microscopy to visualize 12 gene targets in stage-, age- and location-matched chick trunk tissue sections.

CONCLUSIONS: Together, these data demonstrate a robust strategy to acquire and integrate single cell and spatial transcriptomic information, resulting in improved resolution of molecular heterogeneities in complex neural tissue architectures. Successful application of this strategy to the developing SNS provides a roadmap for functional studies of neural connectivity and platform to address complex questions in neural development and regeneration.

Journal Title

Developmental dynamics : an official publication of the American Association of Anatomists

Volume

252

Issue

8

First Page

1130

Last Page

1142

MeSH Keywords

Animals; Transcriptome; RNA, Messenger; In Situ Hybridization, Fluorescence; Sympathetic Nervous System; Ganglia, Sympathetic; Chickens

Keywords

Animals; Transcriptome; RNA, Messenger; In Situ Hybridization, Fluorescence; Sympathetic Nervous System; Ganglia, Sympathetic; Chickens

Comments

Grant support

• 1R21HD106033/HD/NICHD NIH HHS/United States

Recommended Citation

Kasemeier-Kulesa JC, Morrison JA, McKinney S, et al. Cell-type profiling of the sympathetic nervous system using spatial transcriptomics and spatial mapping of mRNA. Dev Dyn. 2023;252(8):1130-1142. doi:10.1002/dvdy.577