Document Type

Article

Publication Date

8-2024

Identifier

DOI: 10.14814/phy2.16168

Abstract

It is not clear as to whether weight bearing and ambulation may affect bone growth. Our goal was to study the role of mechanical loading (one of the components of ambulation) on endochondral ossification and longitudinal bone growth. Thus, we applied cyclical, biologically relevant strains for a prolonged time period (4 weeks) to one tibia of juvenile mice, while using the contralateral one as an internal control. By the end of the 4-week loading period, the mean tibial growth of the loaded tibiae was significantly greater than that of the unloaded tibiae. The mean height and the mean area of the loaded tibial growth plates were greater than those of the unloaded tibiae. In addition, in female mice we found a greater expression of PTHrP in the loaded tibial growth plates than in the unloaded ones. Lastly, microCT analysis revealed no difference between loaded and unloaded tibiae with respect to the fraction of bone volume relative to the total volume of the region of interest or the tibial trabecular bone volume. Thus, our findings suggest that intermittent compressive forces applied on tibiae at mild-moderate strain magnitude induce a significant and persistent longitudinal bone growth. PTHrP expressed in the growth plate appears to be one growth factor responsible for stimulating endochondral ossification and bone growth in female mice.

Journal Title

Physiol Rep

Volume

12

Issue

15

First Page

16168

Last Page

16168

MeSH Keywords

Animals; Female; Parathyroid Hormone-Related Protein; Tibia; Growth Plate; Mice; Weight-Bearing; Stress, Mechanical; Mice, Inbred C57BL; Bone Development; Osteogenesis

Keywords

growth plate; longitudinal bone growth; mechanical loading

Comments

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Publisher's Link: https://physoc.onlinelibrary.wiley.com/doi/10.14814/phy2.16168

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