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The Genetically Engineered Mouse Models (GEMM) core is a CMRI resource created to support investigators in the design, generation, and analysis of novel genetically modified mouse strains in the new state-of-the art vivarium at Children’s Mercy Kansas City. The development of new animal models will provide CMRI researchers an unprecedented tool to investigate the etiology and treatment of pediatric disease. The GEMM core staff have comprehensive expertise in both molecular biology and methods for genetic manipulation of the mouse genome, and the facility is outfitted with the necessary equipment to apply state-of-the-art technologies to our efforts. The GEMM core expertise includes the development, validation, and use of CRISR reagents for efficient genome editing methods in the mouse. In addition to novel model generation the GEMM core offers a host of other support services: cryopreservation of sperm and embryos to protect and archive important strains and storage of these samples in the Children’s Research Institute Biorepository (CRIB), colony maintenance and support for difficult breeding colonies, and importation of mice from outside facilities via in vitro fertilization and rederivation. The GEMM core also supports the design and implementation of an array of genotyping methods, including Transnetyx genotyping services, to maintain the correct allele representation within colonies. These supporting services round out the core's ability to meet the needs of CMRI researchers and to achieve the goal of creating animal models that will advance research in pediatric medicine.
The GEMM core continues to employ and develop cutting edge technology to generate and characterize custom mouse models to address the specific research needs of CMRI investigators. Recently the core staff have developed a genotyping approach that uses Next Generation Sequencing to identify the full gamut of alleles present in CRISPR-edited founder animals. This procedure streamlines what is sometimes a challenging genotyping task and provides a reliable means to select useful founders.
Pence, Laramie and Vivian, Jay L., "Genetically Engineered Mouse Models (GEMM) Core At CMRI" (2022). Research at Children's Mercy Month 2022. 6.