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Publication Date
5-2023
Abstract
Diffuse intrinsic pontine glioma (DIPG) is a highly aggressive brain tumor with rare survival beyond two years. This poor prognosis is largely due to the tumor's highly infiltrative and invasive nature. Nearly 80% of DMGs harbor K27M mutation in the genes encoding histones H3.1 (H3F3A) or H3.3 (HISTIH3B) variants, often with concurrent mutation in ACVR1. Inhibitor of DNA-binding (ID) proteins are key transcriptional regulators of genes involved in lineage commitment and associated with invasiveness and poor clinical outcomes in multiple human cancer. Prior work showed that recurrent K27M and ACVR1 mutations increase ID1 expression in cultured astrocytes, but this has not been confirmed in human tumors or therapeutically targeted. We developed an in-utero electroporation (IUE) murine H3K27M-driven tumor model, which demonstrates increased ID1 expression in H3K27M- and ACVR1-mutated tumor cells. Exome and transcriptome sequencing analysis of multi-focal DMG tumors (n=52) and normal brain tissue revealed that increased ID1 expression is associated with H3K27M/ACVR1-mutation, brainstem location, and correlates with poor survival in patients. ChIP sequencing for H3K27ac and H3K27me3 in multiple DMG tumors (n=5) revealed that ID1 gene is epigenetically active, which matches the epigenetic state of murine prenatal hindbrain cells. Higher ID1-expressing astrocyte-like DIPG cells share a similar transcriptional program with ID1+/SPARCL1+ positive oligo/astrocyte-precursor (OAPC) cells from the developing human brain and demonstrates upregulation of gene sets involved with regulation of cell. Both genetic and pharmacologic [cannabidiol (CBD)] suppression of ID1 results in decreased DIPG cell invasion/migration in vitro and invasion/tumor growth in multiple in vivo models. Mechanistically, CBD reduces proliferation through reactive oxygen species production. Further, DIPG patients treated off-trial with CBD (n=15) showed reduced ID1 expression and improved overall survival. In summary, ID1 is upregulated in DIPG through K27M mediated epigenetic reactivation of a developmental OAPC transcriptional state, and ID1-driven invasiveness of DIPG is therapeutically targetable with CBD.
Document Type
Poster
Recommended Citation
Messinger, Dana; Gibson, Garrett; Harris, Micah K.; Cummings, Jessica R.; Thomas, Chase; Yang, Tao; Sweha, Stefan R.; Woo, Rinette; Siddaway, Robert; Burkert, Martin; Stallard, Stefanie; Qin, Tingting; Mullan, Brendan; Siada, Ruby; Ravindran, Ramya; Niculcea, Michael; Dowling, Abigail; Bradin, Joshua; Ginn, Kevin F.; Gener, Melissa A H; Dorris, Kathleen; Vitanza, Nicholas A.; Schmidt, Susanne V.; Hawkins, Cynthia; Waszak, Sebastian M.; Venneti, Sriram; and Yadav, Viveka Nand, "Therapeutic targeting of prenatal pontine ID1 signaling in DMG" (2023). Research at Children's Mercy Month 2023. 1.
https://scholarlyexchange.childrensmercy.org/research_month2023/1
