Quantification of Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and Testosterone by Liquid Chromatography-Tandem Mass Spectrometry (LC/MS/MS).

Document Type

Article

Publication Date

1-1-2016

Identifier

DOI: 10.1007/978-1-4939-3182-8_29

Abstract

Congenital adrenal hyperplasia (CAH) is a group of autosomal recessive disorders due to enzymatic defects in the biosynthetic pathway of cortisol and/or aldosterone. The analysis of cortisol, 17-hydroxyprogesterone (OHPG), dehydroepiandrosterone (DHEA), 11-deoxycortisol, and testosterone is generally performed in the diagnosis and/or follow-up of CAH. Cortisol is generally analyzed by immunoassays whereas other hormones are preferably assayed by liquid chromatography-tandem mass spectrometry (LC/MS/MS). A multiple reaction monitoring, positive mode atmospheric pressure chemical ionization, LC/MS/MS method is described for the simultaneous quantification of 17-hydroxyprogesterone, DHEA, 11-deoxycortisol, and testosterone. Stable-isotope labeled internal standards are added to serum samples and steroids are extracted by liquid-liquid extraction using methyl tert-butyl ether. The extract is evaporated under stream of nitrogen and the residue is reconstituted in methanol and analyzed by LC/MS/MS.

Journal Title

Methods in molecular biology (Clifton, N.J.)

Volume

1378

First Page

273

Last Page

279

MeSH Keywords

17-alpha-Hydroxyprogesterone; Blood Chemical Analysis; Chromatography, High Pressure Liquid; Cortodoxone; Dehydroepiandrosterone; Statistics as Topic; Tandem Mass Spectrometry; Testosterone; Time Factors

Keywords

11-Deoxycortisol; 17-Hydroxyprogesterone and testosterone; Dehydroepiandrosterone; Tandem mass spectrometry (LC/MS/MS)

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