Quantification of 11-Carboxy-Delta-9-Tetrahydrocannabinol (THC-COOH) in Meconium Using Gas Chromatography/Mass Spectrometry (GC/MS).
Document Type
Article
Publication Date
1-1-2016
Identifier
DOI: 10.1007/978-1-4939-3252-8_11
Abstract
Maternal substance abuse is an ongoing concern and detecting drug use during pregnancy is an important component of neonatal care when drug abuse is suspected. Meconium is the preferred specimen for drug testing because it is easier to collect than neonatal urine and it provides a much broader time frame of drug exposure. We describe a method for quantifying 11-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in meconium. After adding a labeled internal standard (THC-COOH D9) and acetonitrile, samples are sonicated to release both free and conjugated THC-COOH. The acetonitrile/aqueous layer is removed and mixed with a strong base to hydrolyze the conjugated THC-COOH. The samples are then extracted with an organic solvent mixture as part of a sample "cleanup." The organic solvent layer is discarded and the remaining aqueous sample is acidified. Following extraction with a second organic mixture, the organic layer is removed and concentrated to dryness. The resulting residue is converted to a trimethylsilyl (TMS) derivative and analyzed using gas chromatography/mass spectrometry (GC/MS) in selective ion monitoring (SIM) mode.
Journal Title
Methods in molecular biology (Clifton, N.J.)
Volume
1383
First Page
97
Last Page
103
MeSH Keywords
Dronabinol; Female; Gas Chromatography-Mass Spectrometry; Humans; Infant, Newborn; Meconium; Pregnancy; Substance Abuse Detection
Keywords
11-Carboxy-delta-9-tetrahydrocannabinol; Carboxy-THC; Marijuana; Meconium; Substance abuse
Recommended Citation
Peat J, Davis B, Frazee C, Garg U. Quantification of 11-Carboxy-Delta-9-Tetrahydrocannabinol (THC-COOH) in Meconium Using Gas Chromatography/Mass Spectrometry (GC/MS). Methods Mol Biol. 2016;1383:97-103. doi:10.1007/978-1-4939-3252-8_11
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