Leveraging chorionic villus biopsies for the derivation of patient-specific trophoblast stem cells.

Creator(s)

Kaela Varberg, Children's Mercy Kansas CityFollow
Ayelen Moreno-Irusta
Allynson Novoa
Brynne Musser
Joseph M. Varberg
Jeremy P. Goering
Irfan Saadi
Khursheed Iqbal
Hiroaki Okae
Takahiro Arima
John Williams
Margareta D. Pisarska
Michael J. Soares

Document Type

Article

Publication Date

7-1-2025

Identifier

DOI: 10.1038/s42003-025-08393-1; PMCID: PMC12215853

Abstract

Human trophoblast stem (TS) cells are informative in vitro models for the generation and testing of biologically meaningful hypotheses. The goal of this project was to derive patient-specific TS cell lines from clinically available chorionic villus biopsies. Cell outgrowths were captured from human chorionic villus tissue specimens cultured in modified human TS cell medium. Cell colonies emerged early during the culture and cell lines were established and passaged for several generations. Karyotypes of the newly established chorionic villus-derived trophoblast stem (TS^CV) cell lines were determined and compared to initial genetic diagnoses from freshly isolated chorionic villi. Phenotypes of TS^CV cells in the stem state and following differentiation were compared to cytotrophoblast-derived TS (TS^CT) cells. TS^CV and TS^CT cells uniformly exhibited similarities in the stem state and following differentiation into syncytiotrophoblast and extravillous trophoblast cells. Chorionic villus tissue specimens provide a valuable source for TS cell derivation. They expand the genetic diversity of available TS cells and are associated with defined clinical outcomes. TS^CV cell lines provide a new set of experimental tools for investigating trophoblast cell lineage development.

Journal Title

Commun Biol

Volume

8

Issue

1

First Page

964

Last Page

964

MeSH Keywords

Humans; Trophoblasts; Chorionic Villi; Female; Pregnancy; Cell Differentiation; Stem Cells; Biopsy; Cell Line; Chorionic Villi Sampling

PubMed ID

40596474

Keywords

Trophoblasts; Chorionic Villi; Pregnancy; Cell Differentiation; Stem Cells; Biopsy; Cell Line; Chorionic Villi Sampling

Comments

Grants and funding

• R01 HD112559/HD/NICHD NIH HHS/United States
• R01 HD020676/HD/NICHD NIH HHS/United States
• R01 HD099638/HD/NICHD NIH HHS/United States
• F32HD096809/U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
• R01 AI154535/AI/NIAID NIH HHS/United States
• K99 HD115834/HD/NICHD NIH HHS/United States
• K99HD107262/U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
• HD020676/U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
• HD099638/U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
• HD105734/U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
• AI154535/U.S. Department of Health & Human Services | NIH | Office of Research on Women's Health (NIH Office of Research on Women's Health)
• F32 HD096809/HD/NICHD NIH HHS/United States
• R01 HD105734/HD/NICHD NIH HHS/United States
• K99 HD107262/HD/NICHD NIH HHS/United States

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Publisher's Link: https://www.nature.com/articles/s42003-025-08393-1

Recommended Citation

Varberg KM, Moreno-Irusta A, Novoa A, et al. Leveraging chorionic villus biopsies for the derivation of patient-specific trophoblast stem cells. Commun Biol. 2025;8(1):964. Published 2025 Jul 1. doi:10.1038/s42003-025-08393-1